|Bert Menco, PhD|
Ultrastructural Analyses of Chemosensory Signal-Transduction
The main objective our research was to investigate the subcellular sites where olfactory and gustatory (taste) chemosensory signal transduction takes place. Biochemistry, physiology, and light microscopy can give some indication of location of chemosensory molecules, but high-resolution electron microscopy is essential to determine these locations precisely. Chemoreceptor cells have specialized hair like structures that extend in nasal or oral cavities, and that are called cilia and microvilli in olfactory and gustatory chemoreception. We studied whether morphological compartmentalizations parallel functional ones and whether key molecules involved in olfactory signaling are especially contained in these olfactory cilia, and vomeronasal and gustatory microvilli.
• Menco, B. Ph. M. (2005) The fine-structural distribution of G-protein receptor kinase 3, β-arrestin-2, Ca2+/calmodulin-dependent protein kinase II and phosphodiesterase PDE1C2, and a Cl--cotransporter in rodent olfactory epithelia. J. Neurocytol., 34: 11-36. [abstract].
• Menco, B.Ph.M. & E.E. Morrison (2003) Morphology of the mammalian olfactory epithelium: Form, fine structure, function, and pathology. In: Ed., R. L. Doty. Handbook of Olfaction and Gustation, 2nd edition. Marcel Dekker, Inc., New York, pp. 17-49.
• Menco, B.Ph.M., V.McM. Carr, P.I. Ezeh, E.R.
Liman & M.P. Yankova (2001) Ultrastructural localization of G-proteins
and the channel protein
to microvilli of
cells. J. Comp. Neurol., 438: 468-489. [abstract].
• Frederik Ruysch by Jozien van het Reve